Human Linker for activation of T cell ELISA Kit - 96tests

Quantitative Sandwich ELISA for Detection of LAT (Linker for Activation of T Cells) in Human Serum, Plasma, and Cell Culture Supernatants

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• Product Name: Human Linker for Activation of T Cell (LAT) ELISA Kit
• Quantity: 96 Tests
• Sample Types: Serum, Plasma, Cell Culture Supernatants
• Detection Method: Sandwich ELISA
• Detection Wavelength: 450 nm
• Assay Type: Quantitative Immunoassay
• High Sensitivity and Specificity for LAT
• Validated for Multiple Sample Types – Serum, Plasma, Cell Culture Supernatants
• Reliable Quantitative Results – Low intra- and inter-assay variability
•  Easy-to-Use Kit – Pre-coated plates, ready-to-use reagents, and simple protocol
• Stable Storage – Store at 2–8°C for long shelf life (12 months)

The Human Linker for Activation of T Cell (LAT) ELISA Kit is a high-sensitivity immunoassay designed for the quantitative measurement of LAT (Linker for Activation of T Cells) in human biological samples. LAT is an adapter protein crucial for T cell receptor (TCR) signaling and plays an essential role in T cell activation, immune response regulation, and cellular proliferation. LAT is an important target in immunology, autoimmunity, and cancer immunotherapy research.

This kit is ideal for applications such as immune system studies, T cell signaling pathways, and autoimmune disease modeling.

 Test Principle

This assay utilizes a two-site sandwich ELISA format:

1. A capture antibody specific for LAT is pre-coated onto the 96-well microplate.
2. Samples and standards are added; LAT binds to the immobilized antibody.
3. A biotin-conjugated detection antibody is applied to bind to LAT.
4. Streptavidin-HRP binds to the biotinylated detection antibody, and a TMB substrate develops a color proportional to LAT concentration.
5. Absorbance is measured at 450 nm to determine LAT levels in the samples.